|
KMID : 0861020030180040147
|
|
Korea Journal of Herbology
2003 Volume.18 No. 4 p.147 ~ p.156
|
|
|
Studies on a Microtissue of the Puerariae Radix Powder
|
|
安漢圭/Ahn, Han-Kyu
任德彬/李瑛鍾/Lim, Deog-Bin/Lee, Young-Jong
|
|
|
Abstract
|
|
|
Objectives : To examine the morphological differences of Puerariae Radix according to the time of gathering and the original plant, the plants were analyzed and compared through a microscopic examination.
Methods : After collecting the samples according to the time of gathering (April, July, and October) and the original plant (Pueraria lobata Ohwi and Pueraria thomsonii I3enth.1, to make the specimen, the samples were grinded, and the powder was mounted by a solution of Glycerin, Acetic acid and Water at the rate of 1: 1: 1. The specimen was observed by optic microscope (OLYMPUS -BHT, Japan), photographs were taken and then schemes of the characteristics were made.
Results : The following morphological characteristics are the results from a microscopic examination of the Puerariae Radix powder according to the factors such as the original plant and the time of gathering.
1. From a microscopic discernment, the powder samples of uncultivated K o ~ P W I W ~ lobata roots showed no recognizable morphological difference in such factors; suantities of starch contents(starchy, fibrous) and different time of gathering (April, July and October).
1) The powder has a light brown, light yellow or an eggshell color.
2) The starch particles. include the following. The singular grains or granules are forming a spherical, semicircle or a polygonal shape with the diameter of 5-28m. The cleft hilumla are forming a dotted, fissured or star like shape or pattern. The complex grain or granule is composed of 2-10 single pieces of particles
The female starchy Puemria lubata roots collected in July have rather small and few starch particles with the diameter of 5 - 13㎛.
3) The fibers are mostly forming bundles with the diameter of 15-30~. The wall of the fiber, which has become lignified, is thick. Most of the surrounding cells contain the cube (square) crystals of calcium hydroxides and form the crystal fibers. The cell membrane, which contains the cube (square) crystals of calcium hydroxides, has become lignified and thickened. The diameter of cube (square) crystals of calcium hydroxides is 8-23㎛.
4) The bordered pit vessels are rather large with the diameter of 163-600㎛ and mostly broken into pieces. The bordered pits form a hexagonal or an elliptical shape and its disposition is rather tightly arranged.
5 ) The mass of pigment is yellowish brown and forms irregular shapes.
2. The powder samples of cultivated Chinese Pueraria thomsonii roots showed the following morphological characteristics.
1) The powder has a light brown, light yellow or an eggshell color.
2) Many starch particles are observed with the following. The singular grains or granules are forming a spherical, semicircle or a polygonal shape with the diameter of 3-37,m. The cleft hilumla are forming a dotted, fissured or star like shape or pattern. The complex grain or granule is composed of 12-20 single pieces of particles.
3) The fibers are mostly forming bundles with the diameter of 5-25.m. The wall of the fiber, which has become lignified, is thick. Most of the surrounding cells contain the cube (square) crystals of calcium hydroxides and form the crystal fibers. The cell membrane, which contams the cube (square) crystals of calcium hydroxides, has become lignified and thickened. The diameter of cube (square) crystals of calcium hydroxides is 5-17㎛.
4) The diameter of the bordered pit vessels is measured up to 249,m that it is relatively large, And, most of the bordered pit vessels are broken into pieces. The bordered pits form a hexagonal or an elliptical shape and its disposition is rather tightly arranged. Furthermore, some of reticulated tertiary walls have become thickened.
5) The pigment cells contain the unsymmetrical yellowish brown mass of pigments.
Conclusions : From a microscopic discernment, the powder samples of uncultivated Korean Puemrio lobata roots showed no recognizable morphological difference in such factors; quantities of starch contents(starchy, fibrous) and different time of gathering (April, July and October). However, a comparison between the powder samples of Korean Puemria lobata roots and Chinese Puemria thomsonii roots showed some morphological differences in size and quantity of starch particle and the number of single pieces of particles in the complex grain or granule.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|